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Preface… Table of Contents… Contributing Authors.. 1. Vaccine Delivery and Immune Response Basics Andrew Hill, Marie Beitelshees, and Blaine A. Pfeifer 2. Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes Sara Siris, Camilla A. Gladstone, Yanping Guo, Christopher L. Pinder, Robin J. Shattock, Paul F. McKay, Paul R. Langford, and Fadil A. Bidmos 3. Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies Christopher L. Pinder, Paul F. McKay, and Robin J. Shattock 4. Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed Stephen J. Goodswen, Paul J. Kennedy, and John T. Ellis 5. Antigen Discovery in Bacterial Pan-Proteomes Daniel Yero, Oscar Conchillo-Sol, and Xavier Daura 6. Alphavirus-Based Antigen Preparation Kenneth Lundstrom 7. Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells Mihaela-Olivia Dobrica, Catalin Lazar, and Norica Branza-Nichita 8. Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System Julie Harnischfeger, Lukas Ker, Jan Zitzmann, Denise Salzig, and Peter Czermak 9. High-Throughput Process Development for the Chromatographic Purification of Viral Antigens Shaleem I. Jacob, Spyridon Konstantinidis, and Daniel G. Bracewell 10. Production of Zika Virus Virus-Like Particles Atichat Kuadkitkan Suwipa Ramphan, Suchin Worawichawong, Wannapa Sornjai, Nitwara Wikan, and Duncan R. Smith 11. Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs) Kathryn K. Oi, Tom A. Kloter, and Timothy G. Keys 12. Up- and Downstream Processes for Viral Nanoplaxes as Vaccines Keven Lothert, Gregor Dekevic, Daniel Loewe, Denise Salzig, Peter Czermak, and Michael Wolff 13. Whole-Cell Vaccine Preparation: Options and Perspectives| Punit Kumar, Sunita Verma, Kashyap Kumar Dubey, and Pratyoosh Shukla 14. O-Antigen Extraction, Purification, and Chemical Conjugation to a Carrier Protein Francesca Micoli, Carlo Giannelli, and Roberto Di Benedetto 15. Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines Brittany R. Smith and Zhongwu Guo 16. Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines Christa Litschko, Insa Budde, Monika Berger, and Timm Fiebig 17. Attenuation Methods for Live Vaccines Dipasree Hajra, Akshay Datey, and Dipshikha Chakravortty 18. Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism Yasmine Gabal and Joshua D. Ramsey 19. Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells Friederike Eilts, Julie Harnischfeger, Daniel Loewe, Michael Wolff, Denise Salzig, and Peter Czermak 20. Lipofection-Based Delivery of DNA Vaccines Monika Rak, Anna Gra-Sochacka, and Zbigniew Madeja 21. Flavivirus DNA Vaccine Design and Adjuvant Selection Lei Li, Yoshikazu Honda-Okubo, and Nikolai Petrovsky 22. Vaccine Delivery with a Detoxified Bacterial Toxin Diana Diaz-Arvalo, Yanping Chen, and Mingtao Zeng 23. Needle-Less or Non-Invasive Delivery Technology Akshay Datey, Jagadeesh Gopalan, and Dipshikha Chakravortty 24. Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications Viviane Lima Batista, Tales Fernando da Silva, Luis Cludio Lima de Jesus, Ana Paula Tapia-Costa, Mariana Martins Drumond, Vasco Azevedo, and Pamela Mancha-Agresti 25. A Hybrid Biological-Biomaterial Vector for Antigen Delivery Ruiquan Qi, Andrew Hill, and Blaine A. Pfeifer 26. Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens Roozbeh Nayerhoda, Andrew Hill, and Blaine A. Pfeifer 27. Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder Riyad F. Alzhrani, Haiyue Xu, Chaeho Moon, Laura J. Suggs, Robert O. Williams III, and Zhengrong Cui 28. The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers Hui Qian, Yimei Jia, and Michael J. McCluskie 29. Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice Gerard Agbayani, Felicity C. Stark, Bassel Akache, and Michael J. McCluskie 30. The Quantification of Antigen-Specific T Cells by IFN- ELISpot Bassel Akache and Michael J. McCluskie 31. The Measurement of Antigen-Specific IgG Titers by Direct ELISA Bassel Akache, Felicity C. Stark, and Michael J. McCluskie 32. Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model Felicity C. Stark, Renu Dudani, Gerard Agbayani, and Michael J. McCluskie 33. Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils Manmeet Bhalla, Shaunna Simmons, Essi Y.I. Tchalla, and Elsa N. Bou Ghanem 34. Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology Colin G. Hebert, Keya L. Rodrigues, Nicole DiNardo, and Anna-Barbara Hachmann
