Description
Part I : Vectors used in systems neuroscience research Production, testing and verification of Lentivirus for regional targeting in the old-world monkey brain Walter Lerchner, Alyssa Luz-Ricca, Kiana Dash, Violette DerMinassian and Barry J Richmond HiRet/NeuRet vectors: Lentiviral system for highly efficient gene transfer through retrograde axonal transport Kenta Kobayashi, Shigeki Kato, and Kazuto Kobayashi Generation of High-Titer Defective HSV-1 Amplicon Vectors Rachael Neve Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research Masahiro Yamaguchi, Moe Iwata, Riki Kamaguchi, and Fumitaka Osakada Quality control for Adeno-associated viral vector production Ina Ersing, Meghan Rego, Chen Wang, Yijun Zhang, Kate Harten DeMaio, Michelle Tillgren, Alanna Mitsopoulos, Gabrielle Clouse, Marcella Patrick, Karen Guerin, and Melina Fan Part II: Vector modifications and applications Vector tropism Shih-Heng Chen, Bo He, Sarwyn Singh, and Negin P. Martin Viruses for systemic delivery Zikai Wang, Alon Greenbaum, and Jennifer B. Treweek Transcriptomic Definition of Neuron Types Jing He and William R. Stauffer Enhancers for selective targeting Jessica Lin and Jordane Dimidschstein Pathway-selective reversible perturbations using a double-infection technique in the macaque brain Wim Vanduffel and Tadashi Isa Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally-Expressed DREADDs Maricela X Martinez, Mitchell R Farrell, and Stephen V Mahler Part III: Vector delivery and verification of expression Convection-enhanced delivery of viral vectors Devon Griggs, Tiphaine Belloir, Jasmine Zhou, and Azadeh Yazdan-Shahmorad Multi-channel microinjector arrays for efficient viral vector delivery into rhesus monkey brain Nanami L. Miyazaki, Bing Li, and Mark A.G. Eldridge Methods to verify expression and function of DREADDs using PET Yuji Nagai and Takafumi Minamimoto Reporter selection and post-mortem methods to verify transgene expression Kate Heffernan, Yoland Smith and Adriana Galvan Considerations for the use of viral vectors in NHP experiments Martin Bohlen and Sbastien Tremblay
