Description
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species. Part I: Drosophila Protocols 1. Immunostaining of the Developing Embryonic and Larval Drosophila Brain Danielle C. Diaper and Frank Hirth 2. Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila David Jussen and Rolf Urbach 3. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling Karoline F. Kraft and Rolf Urbach 4. Flybow to Dissect Circuit Assembly in the Drosophila Brain Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker 5. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe Benjamin Perruchoud and Boris Egger 6. Using MARCM to Study Drosophila Brain Development Gudrun Viktorin Part II: Other Arthropods 7. Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria George Boyan and Yu Liu Part III: Molluscs 8. Methods in Brain Development of Molluscs Andreas Wanninger and Tim Wollesen Part IV: Xenopus Protocols 9. In Situ Hybridization and Immunostaining of Xenopus Brain Kai-li Liu, Xiu-mei Wang, Zi-long Li, and Ying Liu 10. Microinjection Manipulations in the Elucidation of Xenopus Brain Development Cristine Smoczer, Lara Hooker, Saqib S. Sachani, and Michael J. Crawford 11. Morpholino Studies in Xenopus Brain Development Jennifer E. Bestman and Hollis T. Cline Part V: Zebrafish Protocols 12. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification Gilbert Lauter, Iris Sll, and Giselbert Hauptmann 13. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish Adle Faucherre and Hernn Lpez-Schier 14. Anatomical Dissection of Zebrafish Brain Development Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins Part VI: Chicken Protocols 15. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain Richard P. Tucker and Qizhi Gong 16. Transplantation of Neural Tissue: Quail-Chick Chimeras Andrea Streit and Claudio D. Stern 17. RNAi-Based Gene Silencing in Chicken Brain Development Irwin Andermatt and Esther T. Stoeckli Part VII: Mouse Protocols 18. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development Jinling Liu and Aimin Liu 19. In Utero Electroporation to Study Mouse Brain Development Emilie Pacary and Franois Guillemot 20. The Cre/Lox System to Assess the Development of the Mouse Brain Claudius F. Kratochwil and Filippo M. Rijli
