Description
With significant developments in the areas of chromatography and spectroscopy as well as the unique inherent chemical diversity of natural products, vital in drug research, natural products research has gained new momentum. Fully updating and adding to the previous two editions, Natural Products Isolation, Third Edition documents the latest methods and technologies for natural products isolation with a combination of all new chapters and revised and expanded classic methods. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Natural Products Isolation, Third Edition provides the substantial background information needed by budding natural product researchers as well offering an invaluable reference guide to available methodologies and techniques for the more experienced researchers. 1. An Introduction to Natural Products Isolation Satyajit D. Sarker and Lutfun Nahar 2. Initial and Bulk Extraction of Natural Products Isolation Vronique Seidel 3. Supercritical Fluid Extraction in Natural Products Analyses Lutfun Nahar and Satyajit D. Sarker 4. Accelerated Solvent Extraction (ASE) for Natural Products Isolation Mohammad A. Mottaleb and Satyajit D. Sarker 5. Microwave-Assisted Extraction in Natural Products Isolation Abbas Delazar, Lutfun Nahar, Sanaz Hamedeyazdan, and Satyajit D. Sarker 6. An Introduction to Planar Chromatography and Its Application to Natural Products Isolation Simon Gibbons 7. Isolation of Natural Products by Low-Pressure Column Chromatography Raymond G. Reid and Satyajit D. Sarker 8. Isolation of Natural Products by Ion-Exchange Methods Klaus Dragull and John J. Beck 9. Separation of Natural Products by Countercurrent Chromatography James B. McAlpine, J. Brent Friesen, and Guido F. Pauli 10. Isolation of Natural Products by Preparative High Performance Liquid Chromatography (Prep-HPLC) Zahid Latif and Satyajit D. Sarker 11. Isolation of Natural Products by Preparative Gas Chromatography Temel zek and Fatih Demirci 12. Hyphenated Techniques and Their Applications in Natural Products Analysis Satyajit D. Sarker and Lutfun Nahar 13. Extraction of Plant Secondary Metabolites William P. Jones and A. Douglas Kinghorn 14. Isolation of Marine Natural Products Wael E. Houssen and Marcel Jaspars 15. Isolation of Microbial Natural Products Olov Sterner 16. Extraction and Isolation of Saponins Runner R.T. Majinda 17. Extraction and Isolation of Phenolic Compounds Celestino Santos-Buelga, Susana Gonzalez-Manzano, Montserrat Dueas, and Ana M. Gonzalez-Paramas 18. Scaling-Up of Natural Products Isolation Yash Kumarasamy 19. Follow-Up of Natural Products Isolation Richard J.P. Cannell, Satyajit D. Sarker, and Lutfun Nahar 20. Natural Products Isolation in Modern Drug Discovery Programs Alexander I. Gray, John O. Igoli, and RuAngelie Edrada-Ebel
