Description
Classical natural product chemistry is transitioning to modern day metabolomics as a result of the advent of comprehensive analytical platforms and sensitive analytical instrumentation. Therefore, it is worthwhile to summarize recent developments with current analytical platforms and highlight how metabolomics is being integrated into this classical field to dereplicate and profile natural product extracts. Metabolomics Tools for Natural Product Discoveries: Methods and Protocols aims to unite diverse and recently developed methodologies and protocols in order to identify bioactive secondary metabolites for the purpose of drug discovery. Some topics covered in this volume include applications for the extraction of selected natural products from less common sources such as bryophytes and hard corals, various biological assays, comprehensive applications and strategies for GC-MS, LC-MS, and NMR, as well as protocols and strategies for the structure elucidation of isolated natural products. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Metabolomics Tools for Natural Product Discoveries: Methods and Protocols seeks to serve both professionals and research students with its well-honed methodologies for natural product isolation, biomarker discovery, dereplication, biological assays, and comprehensive metabolomic platforms available for high-throughput analyses. 1. Bryophytes: Liverworts, Mosses and Hornworts – Extraction and Isolation Procedures Yoshinori Asakawa and Agnieszka Ludwiczuk 2. Plant Tissue Extraction for Metabolomics Ute Roessner and Daniel Anthony Dias 3. Detection of Polar Metabolites through the use of Gas Chromatography – Mass Spectrometry David P. De Souza 4. A Robust GC-MS Method for the Quantitation of Fatty Acids in Biological Systems Nirupama Samanmalie Jayasinghe and Daniel Anthony Dias 5. A Workflow from Untargeted LC-MS Profiling to Targeted Natural Product Isolation Damien L. Callahan and Candace E. Elliot 6. Lipidomics -Extraction Protocols for Biological Matrices Thusitha Wasntha Thilanka Rupasinghe 7. Metabolite Analysis of Biological Fluids and Tissues by Proton Nuclear Magnetic Resonance Spectroscopy John Robert Sheedy 8. NMR Spectroscopy: Structure Elucidation of Cycloelatenene A: A Natural Product Case Study Sylvia Urban and Daniel Anthony Dias 9. NMR-Based Metabolomics: A Probe to Utilize Biodiversity Lcia P. Santos Pimenta, Hye Kyong Kim, Robert Verpoorte, and Young Hae Choi 10. Extraction Protocol for Non-Targeted NMR and LC-MS Metabolomics-Based Analysis of Hard Coral and their Algal Symbionts Benjamin R. Gordon, William Leggat, and Cherie Motti 11. Determination of Absolute Configuration Using Single Crystal X-ray Diffraction Abigail L. Albright and Jonathan M. White 12. Natural Product Chemistry in Action: The Synthesis of Melatonin Metabolites K1 and K2 Helmut M. Hgel and Oliver A.H. Jones 13. Discovery, Biosynthesis, and Rational Engineering of Novel Enterocin and Wailupemycin Polyketide Analogues John A. Kalaitziz 14. Bioassays for Anticancer Activities Janice McCauley, Ana Zivanovic, and D. Skropeta 15. Screening for Anti-Diabetic Activities Rima Caccetta and Hani Al Salami 16. Screening for Antibacterial, Antifungal and Anti-Quorum Sensing Activity Elisa J. Hayhoe and Enzo A. Palombo 17. Metabolomics and Dereplication Strategies in Natural Products Ahmed Fares Tawfike, Christina Viegelmann, and RuAngelie Edrada-Ebel 18. Bridging the Gap: Basic Metabolomics Methods for Natural Product Chemistry Oliver A.H. Jones and Helmut M. Hgel 19. Strategies in Biomarker Discovery. Peak Annotation by MS and Targeted LC-MS Micro-Fractionation for de novo Structure Identification by Micro-NMR Philippe J. Eugster, Gatan Glauser, and Jean-Luc Wolfender 20. Statistical Analysis of Metabolomics Data Alysha M. De Livera, Moshe Olshansky, and Terence P. Speed
