Description
This detailed book covers methods for studying, producing, and analyzing therapeutic antibodies, measuring their concentration, developing neutralizing antibodies for them, and for predicting and monitoring their therapeutic efficacy and clinical effects. These biologics are the fastest growing pharmaceutical drug group and have had tremendous clinical and scientific impact in cancer, autoimmune diseases, infectious diseases, and other immune-related diseases, making the content of this volume essential. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methods, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Therapeutic Antibodies: Methods and Protocols serves as an ideal guide for researchers working with the production of, research on, and development of therapeutic antibodies as well as for clinicians using therapeutic antibodies in daily work with patients. 1. Therapeutic Antibodies: An Overview Gunnar Houen 2. Treatment of Autoimmune Diseases with Therapeutic Antibodies: Lessons Learned from PID Patients Allow for Stratification of the Infection Risk Joyce J.B.C. van Beers and Jan G.M.C. Damoiseaux 3. B-Cell Analysis for Monitoring Patients Undergoing B-Cell Depletion for the Treatment of Autoimmune Diseases Jan G.M.C. Damoiseaux, Joyce van Beers, Matthias Busch, and Pieter van Paassen 4. Assessment of Therapeutic Antibody Developability by Combinations of In Vitro and In Silico Methods Adriana-Michelle Wolf Perez, Nikolai Lorenzen, Michele Vendruscolo, and Pietro Sormanni 5. The Therapeutic Antibody Profiler for Computational Developability Assessment Matthew I.J. Raybould and Charlotte M. Deane 6. Short Read-Length Next-Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs Landon Pastushok, Kris Barreto, and C. Ronald Geyer 7. Large Scale Transient Production in ExpiCHO-STM with Enhanced N-Galactosylation-Sialylation and PEI-Based Transfection Xiaotian Zhong, Ashley Schwab, Weijun Ma, Caryl L. Meade, Jing Zhou, Aaron M. D’ Antona, Will Somers, and Laura Lin 8. Production of Therapeutic Single-Chain Variable Fragments (ScFv) in Pichia pastoris Laia Montoliu-Gaya and Sandra Villegas 9. Purification of Therapeutic Antibodies by Protein A Affinity Chromatography Anurag S. Rathore and Sharad Narnaware 10. Ion Exchange Chromatographic Methods for Purification of Therapeutic Antibodies Anurag S. Rathore and Vishwanath Hebbi 11. Structure-Indicated LC-MS/MS Bioanalysis of Therapeutic Antibodies Noriko Iwamoto and Takashi Shimada 12. Use of PASEF for Accelerated Protein Sequence Confirmation and De Novo Sequencing with High Data Quality Detlev Suckau, Waltraud Evers, Eckhard Belau, Stuart Pengelley, Anja Resemann, Wilfred Tang, K. Ilker Sen, Elsa Wagner, Olivier Colas, and Alain Beck 13. Nano-Microscopy of Therapeutic Antibody Aggregates in Solution Yukako Senga, Toshihiko Ogura, Hiroshi Imamura, and Shinya Honda 14. Measuring Self-Association of Antibody Lead Candidates with Dynamic Light Scattering Fabian Dingfelder, Anette Henriksen, Per-Olof Wahlund, Paolo Arosio, and Nikolai Lorenzen 15. Intact Mass Quantitation of Therapeutic Antibodies for Pharmacokinetic Studies Using Immuno-Purification Lisa A. Vasicek, Daniel S. Spellman, and Kevin P. Bateman 16. Detection of Residual Host Cell Proteins in Biotherapeutic Drugs by Concatenated 2D LC-MS/MS Feng Yang, Delia Li, and Donald E. Walker 17. Bioassays for the Evaluation of Target Neutralization and Complement-Dependent Cytotoxicity (CDC) of Therapeutic Antibodies Nohem Salinas-Jazmn, Emilio Medina-Rivero, and Marco Antonio Velasco-Velzquez 18. Methods for Functional Characterization of FcRn Interactions with Therapeutic Antibodies and Fc-Fusion Proteins Shan Chung, Yuwen L. Lin, Van Nguyen, and Chang Liu 19. Method for Measurement of Antibody-Dependent Cellular Phagocytosis Lynn Kamen, Benjamin Ordonia, Srividya Myneni, and Shan Chung 20. Bioluminescent Bridging Immunoassay for Anti-Drug Antibody (ADA) Detection Nidhi Nath, Becky Godat, Rod Flemming, and Marjeta Urh 21. A Surface Plasmon Resonance-Based Assay for Simultaneous Measurement of Serum Concentrations of Therapeutic Antibodies and Anti-Drug Antibodies Marten Beeg, Eleonora Allocati, and Marco Gobbi
