Description
Chronic pain is a complex phenomenon, which continues to remain undertreated in the majority of affected patients thus representing a significant unmet medical need, but the development of cellular, subcellular, and molecular methods of approaching this epidemic of pain shows great promise. In Analgesia: Methods and Protocols, experts in the field present thorough coverage of molecular analgesia research methods from target discovery through target validation and clinical testing to tolerance and dependence, with extensive chapters on emerging receptor classes as targets for analgesic drugs and innovative analgesic strategies. As a volume in the highly successful Methods in Molecular Biology(TM) series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections with tips on troubleshooting and avoiding known pitfalls. Comprehensive and essential, Analgesia: Methods and Protocols promises to aid and enrich the research of all those scientists and clinicians who are interested in what the increasingly molecular future has in store for analgesia research, from the molecular research bench through the animal laboratory to the bedside. 1. Alternatives to Mammalian Pain Models 1: Use of C. elegans for the Study of Volatile Anesthetics Louise M. Steele, Margaret M. Sedensky, and Phil G. Morgan 2. Alternatives to Mammalian Pain Models 2: Using Drosophila to Identify Novel Genes Involved in Nociception Jason C. Caldwell and W. Daniel Tracey, Jr. 3. Animal Models of Acute Surgical Pain Hyangin Kim, Backil Sung, and Jianren Mao 4. Animal Models of Acute and Chronic Inflammatory and Nociceptive Pain Janel M. Boyce-Rustay, Prisca Honore, and Michael F. Jarvis 5. Noxious Heat Threshold Measured with Slowly Increasing Temperatures: Novel Rat Thermal Hyperalgesia Models Kata Blcskei, Gbor Petho, and Jnos Szolcsnyi 6. Locomotor Activity in a Novel Environment as a Test of Inflammatory Pain in Rats David J. Matson, Daniel C. Broom, and Daniel N. Cortright 7. Rationale and Methods for Assessment of Pain-Depressed Behavior in Preclinical Assays of Pain and Analgesia S. Stevens Negus, Edward J. Bilsky, Gail Pereira Do Carmo, and Glenn W. Stevenson 8. Animal Models of Orofacial Pain Asma Khan and Kenneth M. Hargreaves 9. Migraine Models Silvia Benemei, Francesco De Cesaris, Paola Nicoletti, Serena Materazzi, Romina Nassini, and Pierangelo Geppetti 10. Experimental Models of Visceral Pain Mia Karpitschka and Martin E. Kreis 11. Human Correlates of Animal Models of Chronic Pain Arpad Szallasi 12. Human Experimental Pain Models 1: The Ultraviolet Light UV-B Pain Model James Modir and Mark Wallace 13. Human Experimental Pain Models 2: The Cold Pressor Model James Modir and Mark Wallace 14. Human Experimental Pain Models 3: Heat/Capsaicin Sensitization and Intradermal Capsaicin Models James Modir and MarkWallace 15. The Value of the Dental Impaction Pain Model in Drug Development Stephen A. Cooper and Paul J. Desjardins 16. Live Cell Imaging for Studying G Protein-Coupled Receptor Activation in Single Cells Deepak Kumar Saini and Narasimban Gautam 17. Recombinant Cell Lines Stably Expressing Functional Ion Channels Florian Steiner, Sraboni Ghose, and Urs Thomet 18. Ion Channels in Analgesia Research Tamara Rosenbaum, Sidney A. Simon, and Leon D. Islas 19. Electrophysiological and Neurochemical Techniques to Investigate Sensory Neurons in Analgesia Research Alexandru Babes, Michael J.M. Fischer, Gordon Reid, Susanne K. Sauer, Katharina Zimmermann, and Peter W. Reeh 20. The Genetics of Pain and Analgesia in Laboratory Animals William R. Lariviere and Jeffrey S. Mogil 21. RT-PCR Analysis of Pain Genes: Use of Gel-Based RT-PCR for Studying Induced and Tissue-Enriched Gene Expression Kendall Mitchell and Michael J. Iadarola 22. Gene-Based Approaches in the Study of Pathological Pain Elisa Dominguez, Alice Meunier, and Michel Pohl 23. Linkage Analysis and Functional Evaluation of Inherited Clinical Pain Conditions Johannes J. Krupp, Dennis Hellgren, and Anders B. Eriksson 24. Rat Bone Marrow Stromal Cells and Oligonucleotides in Pain Research Mara Florencia Coronel, Norma Alejandra Chasseing, and Marcelo Jos Villar 25. Transplantation of Human Mesenchymal Stem Cells in the Study of Neuropathic Pain Dario Siniscalco 26. Delivery of RNA Interference to Peripheral Neurons in vivo Using Herpes Simplex Virus Anna-Maria Anesti 27. Combination of Cell Culture Assays and Knockout Mouse Analyses for the Study of Opioid Partial Agonism Soichiro Ide, Masabumi Minami, Ichiro
