Description
Antimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of antibiotic issues and answer the worldwide demand for novel antibiotics therapies. Beginning with a review chapter that guides the reader through the worldwide demand for novel antibiotics therapies, the volume continues with sections covering new screening procedures and environmental sources, advances in analytical, microbiological, and biotechnological methodologies, antibiotic production and antibiotic resistances, as well as considerations of drug trials and clinical concerns regarding multi-resistant patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antimicrobial Therapies: Methods and Protocols provides a reference source for health, laboratory, and industrial professionals, as well as for graduate students in a number of bio-sanitary disciplines, including medicine, nursery, biotechnology, veterinary, microbiology, genetics, molecular biology, nutrition, farming, and more. Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com. Part I: General Overview 1. Worldwide Clinical Demand for Antibiotics: Is It a Real Countdown? Carlos Barreiro and Jos-Luis Barredo Part II: Antibiotic Screening 2. Collection, Extraction, and In Vitro Antibacterial Evaluation of Plants Used in Traditional Medicine Franois Chassagne and Cassandra Leah Quave 3. Microbial Isolation and Characterization of New Antibiotic-Producer Strains from Decayed Wood scar Velasco-Rodrguez, Mariana Fil, Laura Garca-Calvo, Katarina Kosalkov, and Carlos Barreiro 4. Characterization of Extremophilic Actinomycetes Strains as Sources of Antimicrobial Agents Diyora Abdukhakimova, Zhannur Markhametova, Saikal Shamkeeva, Ainur Zhulamanova, Lyudmila Trenozhnikova, Vladimir Berezin, and Azliyati Azizan 5. Dilution-To-Extinction Platform for the Isolation of Marine Bacteria Producing Antitumor Compounds Xulio Bentez, Jess Garca, Elena G. Gonzlez, and Fernando de la Calle Part III: Methodologies 6. Screening Systems for Stable Markerless Genomic Deletions/Integrations in Streptomyces Species Jan Kormanec, Bronislava Rezuchova, and Renata Novakova 7. Antibacterial Assay in a Screening Project Francisco Romero and Antonio Fernndez 8. Screening of Antibiotic Gene Clusters in Microorganisms Isolated from Wood Mariana Fil, scar Velasco-Rodrguez, Laura Garca-Calvo, Alberto Sola-Landa, and Carlos Barreiro 9. Methods in Screening Antiviral Drugs Against Enterovirus 71 Nadia Abubakar Omar Abubakar Bajaber and Babu Ramanathan 10. High-Efficiency Electroporation for Genetic Improvement of Fungal Strains Jessica Cruz-Ramn, Francisco J. Fernndez, and Francisco Fierro 11. Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC Katarina Kosalkov, Isabel-Clara Snchez-Orejas, Laura Cueto, and Carlos Garca-Estrada 12. Screening Fermentation and Extract Generation Francisco Romero and Antonio Fernndez 13. A Simple Antifungal Assay for Testing Actinomycetes and Other Microbial Extracts Mara A. Vinuesa and Antonio Fernndez 14. A User Guide for the Identification of New RiPP Biosynthetic Gene Clusters Using a RiPPER-Based Workflow Alaster D. Moffat, Javier Santos-Aberturas, Govind Chandra, and Andrew W. Truman 15. Novel Methods to Identify Oxidative Stress-Producing Antibiotics lvaro Mourenza, Jos A. Gil, Lus M. Mateos, and Michal Letek 16. Coupled Transcriptomics for Differential Expression Analysis and Determination of Transcription Start Sites: Design and Bioinformatics Antonio Rodrguez-Garca, Alberto Sola-Landa, and Rosario Prez-Redondo 17. High Throughput Screening Assays for Dengue Antiviral Drug Development Shobana Gabriel Jabanathan, Low Zhao Xuan, and Babu Ramanathan 18. Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline Lucija Slemc, spela Pikl, Hrvoje Petkovic, and Martina Avbelj Part IV: Antibiotic Production 19. Production, Detection, Extraction, and Quantification of Polyene Antibiotics Eva G. Barreales and Jess F. Aparicio 20. Strain Improvement Program of Streptomyces roseosporus for Daptomycin Production Sara Rubio, Sonia Martnez-Cmara, Juan Luis de la Fuente, Marta Rodrguez-Siz, and Jos-Luis Barredo Part V: Antibiotic Resistance 21. Comparative Metabolomics Revealing the Metabolic Responses of Pathogenic Bacteria to Different Antibiotics Fouad Choueiry, Xiaowei Sun, and Jiangjiang Zhu 22. Proteomic Analysis of Antibiotic Susceptibility in Enterobacteriaceae Sarah L. Keasey, Bryan M. Zhao, Robert G. Ulrich, and Moo-Jin Suh 23. Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose-Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi) Samantha Donnellan, Carmen Martnez-Rodrguez, Ghaith Aljayyoussi, and Giancarlo A. Biagini Part VI: Market 24. Clinical Drug Trials: The Path to the Patient Cristina Diez Pascual 25. Antibiotics Therapy in the Multi-Resistant Patient Marta Mara Blanco-Nistal and Jess Antonio Fernndez-Fernndez
