Description
This detailed volume provides a single, valuable reference source for methods that definitively identify and accurately quantify apoptosis. The book begins with common methods utilized to detect and quantitate apoptosis, as well as apoptosis signaling pathways in toxicological and other related research. It continues with multi-parametric and phased apoptosis assays for detecting early and late apoptosis or distinguishing apoptosis from necrosis and autophagy. Subsequent chapters focus on recent advances in real time and high-throughput assays that detect and quantitate apoptosis and apoptosis signaling pathways. Final chapters focus on recent developments in preclinical anticancer therapeutics targeting apoptosis. Written for the Methods in Pharmacology and Toxicology series, chapters feature step-by-step descriptions of the methodologies, as well as expert tips and implementation advice. Vital and authoritative, Apoptosis Methods in Toxicology serves novice scientists as well as experts, utilizing a range of instruments from common laboratory equipment to high-end expensive and automated machinery capable of performing real time apoptotic measurements. 1. An Overview of Apoptosis Methods in Toxicological Research: Recent Updates Perpetua M. Muganda 2. Detection of Apoptosis: From Bench Side to Clinical Practice William P. Bozza, Julianne D. Twomey, Su-Ryun Kim, and Baolin Zhang 3. Determining the Extent of Toxicant-Induced Apoptosis Using Concurrent Phased Apoptosis Assays Akamu Jude Ewunkem and Perpetua M. Muganda 4. Measurement of Apoptosis by Multiparametric Flow Cytometry William Telford 5. Detecting Apoptosis, Autophagy, and Necrosis Jack Coleman, Rui Liu, Kathy Wang, and Arun Kumar 6. A Low-Cost Method for Tracking the Induction of Apoptosis Using FRET-Based Activity Sensors in Suspension Cells Akamu J. Ewunkem, Carl D. Parson II, Perpetua M. Muganda, and Robert H. Newman 7. FRET-Based Measurement of Apoptotic Caspase Activities by High-Throughput Screening Flow Cytometry Christian T. Hellwig, Agnieszka H. Ludwig-Galezowska, and Markus Rehm 8. Automated Ratio Imaging Using Nuclear-Targeted Fret Probe-Expressing Cells for Apoptosis Detection Krupa Ann Mathew, Deepa Indira, Jeena Joseph, Prakash Rajappan Pillai, Indu Ramachandran, Shankara Narayanan Varadarajan, and Santhoshkumar Thankayyan Retnabai 9. Antibody-Based Proteomic Analysis of Apoptosis Signaling Matthew P. Stokes, Hongbo Gu, and Jeffrey C. Silva
