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Series Preface… Preface… Table of Contents… Contributing Authors… 1. Combined In Situ Hybridization Chain Reaction and Immunostaining to Visualize Gene Expression in Whole-Mount Drosophila Central Nervous Systems Julia C. Duckhorn, Ian P. Junker, Yun Ding, and Troy R. Shirangi 2. Mapping Brain-Wide Mesoscale Connectome from Single Animals with BRICseq Longwen Huang 3. Visualization of Mating-Dependent Activation of Neurons and Oogenesis in Drosophila melanogaster Yuto Yoshinari and Ryusuke Niwa 4. Measuring Sleep in Drosophila Takaaki Miyazaki, Julie A. Williams, and Hirofumi Toda 5. Gut Microbes and Drosophila Behavior Hibiki Kadoguchi, Aki Hori, and Takayuki Kuraishi 6. Measurement of Thermoregulatory Behavior in Drosophila melanogaster Takuto Suito, Kohjiro Nagao, Masahiro Kai, Naoto Juni, Takaaki Sokabe, and Masato Umeda 7. Quantifying Social Interactions in Medaka Fish Saori Yokoi, Yasuko Isoe, Mu-Yun Wang, Masahiro Daimon, Teruhiro Okuyama, and Hideaki Takeuchi 8. A Method for Selective Breeding to Domesticate Mice Bhim B. Biswa and Tsuyoshi Koide 9. Bioassaying the Function of Pheromones in Drosophila melanogaster’s Social Behavior Thomas A. Verschut, Philip Kohlmeier, and Jean-Christophe Billeter 10. Miniature Microscopy of Hippocampal CA1 to Identify Engram Cells and Record Calcium Transients for Analyses of Ensemble Activities Noriaki Ohkawa and Khaled Ghandour 11. Optical Recording of Brain Neuron Activities from a Male Drosophila Behaving on a Treadmill Soh Kohatsu and Daisuke Yamamoto 12. In Vivo Brain Imaging in Freely Moving and Socially Interacting Drosophila Dhruv Grover, Takeo Katsuki, and Ralph J. Greenspan 13. Drosophila as a Model to Explore Individuality Kuo-Ting Tsai and Ya-Hui Chou 14. Automated Behavior Analysis using a YOLO-Based Object Detection System Kai Amino and Takashi Matsuo 15. Proteomic Analysis of C. elegans Neurons using TurboID-Based Proximity Labeling Murat Artan and Mario de Bono Subject Index List…




