Description
Cancer Vaccines: Methods and Protocols explores the manipulation and modification of immune cells, the manipulation and modification of tumor cells as well as the manipulation of immune/tumor interactions and various delivery mechanisms, with the overall end goal of evoking a tumor-specific response and overcoming the immuno-evasive mechanisms employed by the tumor cells. This detailed volume also covers the subject of cancer vaccines in a more global sense with its section on the advances, challenges, and future of cancer vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Cancer Vaccines: Methods and Protocols aims to help guide researchers toward developing further generations of cancer vaccines that are both safe and efficacious, with the hope that cancer vaccines will be the standard of care in the very near future. Part I: Manipulation and Modification of Immune Cells: Dendritic Cells 1. Single Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumor-Associated Antigen and a TriMix of Costimulatory Molecules Daphn Benteyn, An M.T. Van Nuffel, Sofie Wilgenhof, and Aude Bonehill 2. Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccine Hyun-Ju Lee, Nu-Ri Choi, Manh-Cuong Vo, My-Dung Hoang, Youn-Kyung Lee, and Je-Jung Lee 3. Pulsing Dendritic Cells with Whole Tumor Cell Lysates Laura Alaniz, Manglio M. Rizzo, and Guillermo Mazzolini 4. Antigen Trapping by Dendritic Cells for Anti-Tumor Therapy Chiranjib Pal 5. Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens Manglio M. Rizzo, Laura Alaniz, and Guillermo Mazzolini 6. Tumor Antigen/Cytokine-Pulsed Dendritic Cells in Therapy Against Lymphoma Sumit K. Hira, Deepak Verma, and Partha P. Manna 7. Dendritic Cells Primed with Protein-Protein Fusion Adjuvant Liying Wang and Yongli Yu 8. Antigen-Specific mRNA Transfection of Autologous Dendritic Cells Fabian Benencia 9. Electroporation of Dendritic Cells with Autologous Total RNA from Tumor Material Francesca Milano and K.K. Krishnadath 10. Dendritic Cells Transfected with Adenoviral Vectors as Vaccines Joseph Senesac, Dmitry Gabrilovich, Samuel Pirruccello, and James E. Talmadge 11. Genetic Modification of Dendritic Cells with RNAi Xiao-Tong Song 12. Fast Monocyte-Derived Dendritic Cells-Based Immunotherapy Gamal Ramadan 13. Intratumoral Injection of BCG-CWS Pretreated Dendritic Cells Following Tumor Cryoablation Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, and Yutaka Kawakami 14. Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccines Roeland Lameris, Famke L. Schneiders, Tanja D. de Gruijl, and Hans J. van der Vliet Part II: Manipulation and Modification of Immune Cells: T Lymphocytes and NK Cells 15. Modification of T Lymphocytes to Express Tumor Antigens Aaron E. Foster and Xiao-Tong Song 16. Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptor Liza B. John, Tess M. Chee, David E. Gilham, and Phillip K. Darcy 17. Genetic Modification of Cytotoxic T Lymphocytes to Express Cytokine Receptors Serena K. Perna, Barbara Savoldo, and Gianpietro Dotti 18. Monitoring the Frequency and Function of Regulatory T Cells and Summary of the Approaches Currently Used to Inhibit Regulatory T Cells in Cancer Patients Chiara Camisaschi, Marcella Tazzari, Licia Rivoltini, and Chiara Castelli 19. Cytokine Activation of Natural Killer Cells Syh-Jae Lin, Pei-Tzu Lee, and Ming-Ling Kuo Part III: Manipulation and Modification of Tumor Cells 20. Loading of Acute Myeloid Leukemia Cells with Poly(I:C) by Electroporation Eva Lion, Charlotte M. de Winde, Viggo F.I. Van Tendeloo, and Evelien L.J.M. Smits 21. Autologous Tumor Cells Engineered to Express Bacterial Antigens Vijayakumar K. Ramiya, Maya M. Jerald, Patricia D. Lawman, and Michael J.P. Lawman 22. Tumor Cell Transformation Using Antisense Oligonucleotide Mohamed R. Akl and Nehad M. Ayoub 23. The Direct Display of Costimulatory Proteins on Tumor Cells as a Means of Vaccination for Cancer Immunotherapy Haval Shirwan, Esma S. Yolcu, Rajesh K. Sharma, Hong Zaho, and Orlando Grimany-Nuno Part IV: Manipulation of Immune/Tumor Interactions 24. Cloning Variable Region Genes of Clonal Lymphoma Immunoglobulin for Generating Patient-Specific Idiotype DNA Vaccine Soung-chul Cha, Hong Qin, Ippei Sakamaki, and Larry Kwak 25. Heat Shock Proteins Purified from Autologous Tumors Using Antibody-Based Affinity Chromatography Christian Kleist, Marco Randazzo, Janina Jiga, and Peter Terness 26. Invariant Chain-Peptide Fusion Vaccine Using HER-2/neu Sonia A. Perez, George E. Peoples, Michael Papamichail, and Constantin N. Baxevanis 27. TLR-9 Agonist Immunostimulatory Sequence Adjuvants Linked to Cancer Antigens Hidekazu Shirota and Dennis M. Klinman 28. Production of Multiple CTL Epitopes from Multiple Tumor-Associated Antigens Rena Morita, Yoshihiko Hirohashi, Munehide Nakatsugawa, Takayuki Kanaseki, Toshihiko Torigoe, and Noriyuki Sato 29. Preparation of Polypeptides Comprising Multiple TAA Peptides Bing Ni, Zhengcai Jia, and Yuzhang Wu 30. Idiotype Vaccine Production Using Hybridoma Technology Susana Inoges, Ascension Lopez-Diaz de Cerio, Helena Villanueva, Fernando Pastor, and Maurizio Bendandi 31. Preparation of Cancer-Related Peptide Cocktails that Target Heterogeneously-Expressed Antigens Reshu Gupta and Pradip P Sachdeva Part V: Delivery Mechanisms 32. Making an Avipoxvirus Encoding a Tumor-Associated Antigen and a Costimulatory Molecule Paul M. Howley, Kerrilyn R. Diener, and John D. Hayball 33. Bacterial Vectors for the Delivery of Tumor Antigens Yan Wang, Bertrand Toussaint, and Audrey Le Goullec 34. Preparation of Peptide Microspheres Using Tumor Antigen-Derived Peptides Santwana Bhatnagar, Raza Ali Naqvi, Riyasat Ali, and D.N. Rao 35. Production of Antigen-Loaded Biodegradable Nanoparticles and Uptake by Dendritic Cells Vijaya Bharti Joshi, Sean M. Geary, and Aliasger K. Salem 36. Development of Plasmid-Lipid Complexes for Direct Intratumoral Injection Rama P. Kotipatruni and Ganji Purnachandra Nagaraju Part VI: The Advances, Challenges, and Future of Cancer Vaccines 37. The Use of Dendritic Cells for Peptide-Based Vaccination in Cancer Immunotherapy Mohamed L. Salem 38. Advances in Host and Vector Development for the Production of Plasmid DNA Vaccines Juergen Mairhofer and Alvaro R. Lara 39. Challenges Facing the Development of Cancer Vaccines Mayer Fishman 40. Future of Cancer Vaccines Hauke Winter, Bernard A. Fox, and Dominik Rttinger
