Description
DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines. Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease. Part I: DNA Vaccine Design and Enhancement 1. A Blueprint for DNA Vaccines Design Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi 2. Enhancement of Plasmid-Mediated Transgene Expression Daniela Fioretti, Sandra Iurescia, and Monica Rinaldi 3. Strategies for Improving DNA Vaccines Performance Sandra Iurescia, Daniela Fioretti, and Monica Rinaldi 4. Enhancement of DNA Vaccine Efficacy by Intracellular Targeting Strategies Elisabete Borges Freitas, Ana Margarida Henriques, Miguel Fevereiro, Duarte Miguel Prazeres, and Gabriel Amaro Monteiro 5. Progresses in DNA-Based Heterologous Prime-Boost Immunization Strategies Ronald J. Jackson, David B. Boyle, and Charani Ranasinghe 6. Development of Antibiotic-Free Selection System for Safer DNA Vaccination Jeremy M. Luke, Aaron E. Carnes, and James A. Williams Part II: Delivery System 7. Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin Anita Gothelf and Julie Gehl 8. DNA Vaccination in Skin Enhanced by Electroporation Kate E. Broderick, Amir S. Khan, and Niranjan S. Sardesai 9. Intradermal Vaccination by DNA Tattooing Joost H. van den Berg, Koen Oosterhuis, Ton N.M. Schumacher, John B.A.G. Haanen, and Adriaan D. Bins 10. Microneedle Applications for DNA Vaccine Delivery to the Skin Hae-yong Seok, Hyemee Suh, Sunghyun Baek, and Yeu-Chun Kim 11. Multivalent DNA-Based Vectors for DNA Vaccines Delivery Young Hoon Roh, Kwang Lee, Jessica Jane Ye, and Dan Luo 12. Superparamagnetic Nanoparticles Delivery of DNA Vaccine Fatin Nawwab Al-Deen, Cordelia Selomulya, Charles Ma, and Ross L. Coppel Part III: Production, Purification, and Quality 13. Plasmid Fermentation Process for DNA Immunization Applications Aaron E. Carnes and James A. Williams 14. Pharmaceutical Grade Large Scale Plasmid DNA Manufacturing Process Marco Schmeer and Martin Schleef Part IV: New Vaccine Applications 15. Protective and Therapeutic DNA Vaccination Against Allergic Diseases Almedina Isakovic, Richard Weiss, Josef Thalhamer, and Sandra Scheiblhofer 16. Immunotherapy for Alzheimer’s Disease: DNA- and Protein-Based Epitope Vaccines Hayk Davtyan, Irina Petrushina, and Anahit Ghochikyan 17. Tetravalent DNA Vaccine Product as a Vaccine Candidate Against Dengue Kevin R. Porter, Nimfa Teneza-Mora, and Kanakatte Raviprakash 18. DNA Vaccination as a Treatment for Chronic Kidney Disease Yuan Min Wang and Stephen I. Alexander
