Description
Part I: African killifish (Nothobranchius furzeri) 1. Establish a fishroom for African turquoise killifish Nothobranchius furzeri Michelle B. Vilensky and Chi-Kuo Hu 2. Gene editing and transgenic reporter assays in African killifish Wei Wang 3. Spinal cord injury in African killifish Yufei Lou and Wei Wang 4. Whole brain clearing and immunolabelling in the African killifish N. furzeri Augusto Ortega Granillo, Fengyan Deng, Hannah Wilson, Yongfu Wang, and Alejandro Snchez Alvarado 5. Studying Mating Behaviors in Nothobranchius furzeri Bernadette D. Johnson and Adam G. Jones 6. A chromatin immunoprecipitation (ChIP) protocol for African turquoise killifish Jiaxing Jin and Deqing Hu 7. EdU labeling of proliferating cells in the African killifish Nothobranchius furzeri Augusto Ortega Granillo, Fengyan Deng, Yongfu Wang, Wei Wang, Alejandro Snchez Alvarado 8. Immunofluorescence staining on cryosections with high autofluorescence in African killifish Shiqi Liu and Wei Wang 9. Rapid visualization of gene expression using chromogenic RNA in situ hybridization in African killifish Mengjun Liu, Shiqi Liu, and Wei Wang 10. Profiling gene expression in African turquoise killifish Nothobranchius furzeri embryos with RNA fluorescence in situ hybridization chain reaction (HCR) Lu Jia and Chi-Kuo Hu 11. Cell dissociation of tissues for single-cell analysis in African killifish Liping Zhang and Wei Wang Part II: Mexican cavefish (Astyanax mexicanus) 12. Cryopreservation of the Mexican Tetra: Astyanax mexicanus Diana P. Baumann and Andrew Ingalls 13. Whole-mount multicolor fluorescent labeling by in situ hybridization in Astyanax mexicanus embryos and larvae Maryline Blin, Sylvie Rtaux, and Jorge Torres-Paz 14. Application of CRISPR-Cas9 for functional analysis in A. mexicanus Itzel Sifuentes-Romero, Estephany Ferrufino, and Johanna E. Kowalko 15. Immunohistochemistry and in vivo neural imaging in A. mexicanus Evan Lloyd, Robert A. Kozol, Erik R. Duboue, and Alex C. Keene 16. Sleep behavior analysis in Astyanax mexicanus Pierce Hutton, Evan Lloyd, Madelyn Dotson, and Alex C. Keene 17. A new method to analyze non-visual based social-like interactions in asocial cavefish Motoko Iwashita and Masato Yoshizawa 18. A generalist’s guide to quantifying swimming behavior within traditional and non-traditional teleost models Luke Olsen, Richard Alexander, Jaya Krishnan, and Nicolas Rohner 19. Recording acoustic behaviour in Astyanax mexicanus fish: acquisition, decryption, interpretation Carole Hyacinthe Part III: Mini-brain 20. Generation of mini-brains from hiPSCs Yan Wang, Dai Tsuchiya and Chongbei Zhao 21. Generating Human Pluripotent Stem Cell-Derived AssemBloids(TM) to Model Interneuron Migration and Immune Cell Interactions Negin Imani Farahani, Jeanne Chan, Adam Aonuevo, Leon H. Chew, and Erin Knock 22. Multiplexed quantitative proteomics analysis of developing human brain organoids Laurence Florens, Anita Saraf, Yan Hao, Ying Zhang, Yan Wang, Tari J. Parmely, Chongbei Zhao, and Michael P. Washburn
