Description
The evaluation of potential mutagenic activity is a critical step in the assessment of the safety of both new and pre-existing chemical types. In Genetic Toxicology: Principles and Methods, expert contributors help to satisfy the demand for education in this tremendously important area of study. The volume covers three basic areas: the scientific basis of the discipline, the methodologies of the main test assays, and the application of the methods, all aimed primarily at scientists in the safety departments of the industries working with both natural and synthetic chemicals. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Intuitive and cutting-edge, Genetic Toxicology: Principles and Methods provides crucial support to both laboratory workers in providing quality information on the appropriate application of techniques and to study directors in their assay selection and protocol design in this vital field. 1. The Application of Structure Activity Relationships to the Prediction of the Mutagenic Activity of Chemicals Philip Judson 2. Bacterial Mutagenicity Assays: Test Methods David Gatehouse 3. The Mouse Lymphoma Assay Melvyn Lloyd and Darren Kidd 4. Mammalian Cell HPRT Gene Mutation Assay: Test Methods George E. Johnson 5. The In Vitro Mammalian Chromosome Aberration Test Gill Clare 6. The Interpretation and Analysis of Cytogenetic Data Natalie Danford 7. The In Vitro Micronucleus Assay Ann T. Doherty 8. The In Vitro and In Vivo Comet Assays Brian Burlinson 9. Assessment of DNA Interstrand Crosslinks (ICLs) Using the Modified Alkaline Comet Assay Jian Hong Wu and Nigel J. Jones 10. 32P-Postlabelling for the Sensitive Detection of DNA Adducts Nigel J. Jones 11. Methods for the Detection of DNA Adducts Karen Brown 12. The GADD45a-GFP GreenScreen HC Assay Richard M. Walmsley and Matthew Tate 13. Real Time Reverse-Transcription Polymerase Chain Reaction (RT-PCR): Technical Considerations for Gene Expression Analysis Shareen H. Doak and Zoulikha M. Zar 14. Cytogenetic In Vivo Assays in Somatic Cells Ann T. Doherty, Adolph Baumgartner, and Diana Anderson 15. Cytogenetic Methods in Human Biomonitoring: Principles and Uses Raluca A. Mateuca, Ilsa Decordier, and Micheline Kirsch-Volders 16. The Measurement of Induced Genetic Change in Mammalian Germ Cells Ilse-Dore Adler, Francesca Pacchierotti, and Antonella Russo 17. Transgenic Animal Mutation Models: A Review of the Models and How They Function Steve Dean 18. Analysis of Genotoxicity Data in a Regulatory Context Ian de G. Mitchell and David O.F. Skibinski
