Description
This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry. Preface… Table of Contents…Contributing Authors… 1. Liposomes Came First: The Early History of LiposomologyVolkmar Weissig2. Thin Film Hydration followed by Extrusion Method for Liposome PreparationHongwei Zhang3. Preparation of DRV LiposomesSophia G. Antimisiaris4. Method of Simultaneous Analysis of Liposome Components by HPTLC/FIDSophia Hatziantoniou and Costas Demetzos5. Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution ScaleBrigitte Papahadjopoulos-Sternberg6. Liposome Formulations of Hydrophobic DrugsReto A. Schwendener and Herbert Schott7. A “Dock and Lock” Approach to Preparation of Targeted LiposomesMarina V. Backer and Joseph M. Backer8. Coupling of Ligands to the Liposome Surface by Click ChemistryMaria Vittoria Spanedda, Marcella De Giorgi, Fatouma Sad Hassane, Francis Schuber, Line Bourel-Bonnet, and Benot Frisch9. Elastic Liposomes for Topical and Transdermal Drug DeliveryMelani A. Solomon11. Environmental Scanning Electron Microscope Imaging of Vesicle SystemsYvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal U. R. Mohammed, Sarah E. McNeil, and Anil Vangala12. Liposome Biodistribution via Europium ComplexesNathalie Mignet and Daniel Scherman13. Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Preformed Liposomes for Diagnostic Imaging and Radionuclide Therapy
