Description
Studies of membrane transporters have had a great impact on our understanding of human diseases and the design of effective drugs. In Membrane Transporters in Drug Discovery and Development: Methods and Protocols, expert researchers provide practical methodologies of the ongoing research on membrane transporters, considering applications of transporter technologies in drug discovery and development. Chapters include new and useful fields and methodologies, including pharmacogenomics, nutrigenomics, systems biology, bioinformatics, nuclear magnetic resonance (NMR), imaging, and quantitative real-time-PCR. Transporter studies in drug discovery and development for various diseases are discussed, including neuropsychiatric disorders, cardiovascular diseases, ophthalmic diseases, cancer, and diabetes. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Wide-ranging and current, Membrane Transporters in Drug Discovery and Development: Methods and Protocols delivers a collection of practical protocols that can be used immediately in the lab, along with critical surveys of key topics by leading researchers in the field. Membrane Transporters in Drug Discovery and Development: Methods and Protocols Table of Contents: Table of Contents Preface Contributors 1. Membrane Transporters and Drug Development: Relevance to Pharmacogenomics, Nutrigenomics, Epigenetics, and Systems Biology Qing Yan 2. Bioinformatics for Transporter Pharmacogenomics and Systems Biology: Data Integration and Modeling with UML Qing Yan 3. Multi Drug Resistance: Phylogenetic Characterization of Superfamilies of Secondary Carriers that Include Drug Exporters Ming Ren Yen, Jonathan Chen, Jose L. Marquez, Eric I. Sun and Milton H. Saier, Jr. 4. Targeting Drug Transporters – Combining in Silico and In Vitro Approaches to Predict in Vivo Praveen M. Bahadduri, James E. Polli, Peter W. Swaan and Sean Ekins 5. Methods to Evaluate Transporter Activity in Cancer Keisuke Mitsuoka, Douglas D. Ross, and Takeo Nakanishi 6. Analysis of Expression of Drug Resistance-Linked ABC Transporters in Cancer Cells by Quantitative RT-PCR Anna Maria Calcagno and Suresh V. Ambudkar 7. Fluorescence Studies of Drug Binding and Translocation by Membrane Transporters Frances J. Sharom, Ronghua Liu and Balpreet Vinepal 8. A Model for Transport Studies of the Blood-Brain Barrier Dennis J. Bobilya 9. Genetic Variants in the Vesicular Monoamine Transporter 1 (VMAT1/SLC18A1) and Neuropsychiatric Disorders Falk W. Lohoff 10. Equilibrium Binding and Transport by Vesicular Acetylcholine Transporter Parul Khare, Aubrey R. White, Anuprao Mulakaluri, and Stanley M. Parsons 11. ABC Transporters in Ophthalmic Disease Corey Westerfeld 12. Imaging of Protein Translocation insitu in Skeletal Muscle of Living Mice Hans P.M.M Lauritzen 13. Glucose Transporters in Parasitic Protozoa Scott M. Landfear 14. NMR Studies of Membrane Proteins Gabriel A. Cook and Stanley J. Opella 15. Site-Directed Mutagenesis in the Study of Membrane Transporters Audra A. McKinzie, Renae M. Ryan, and Robert J. Vandenberg 16. Xenopus laevis Oocytes Stefan Brer 17. Measurement of Intracellular pH Frederick B. Loiselle and Joseph R. Casey 18. Measurement of Plasma Membrane Calcium-Calmodulin Dependent ATPase (PMCA) Activity Tamer M. A. Mohamed, Florence M. Baudoin-Stanley, Riham Abou-Leisa, Elizabeth Cartwright, Ludwig Neyses, and Delvac Oceandy 19. Assessment of the Contribution of the Plasma Membrane Calcium ATPase, PMCA, Calcium Transporter to Synapse Function Using Patch Clamp Electrophysiology and Fast Calcium Imaging Chris J. Roome and Ruth M. Empson
