Description
This thorough volume explores the possibility of detecting and identifying toxigenic fungi, able to produce secondary metabolites known as mycotoxins, which cause severe health problems in humans and animals after exposure to contaminated food and feed, having a broad range of toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity. Beginning with a section on fungal genera and species of major significance along with their associated mycotoxins, the book continues with sections on Polymerase Chain Reaction (PCR)-based methods for the detection and identification of mycotoxigenic fungi, PCR-based methods for multiplex detection of mycotoxigenic fungi, as well as sections on combined approaches and new methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mycotoxigenic Fungi: Methods and Protocols will aid researchers working in this vital field to provide insight into possible actions to reduce mycotoxin contamination of crop plants and the food/feed byproducts. Part I: Fungal Genera and Species of Major Significance and Their Associated Mycotoxins 1. Mycotoxins: An Underhand Food Problem Antonio Moretti, Antonio F. Logrieco, and Antonia Susca 2. Alternaria Species and Their Associated Mycotoxins Virginia Elena Fernndez Pinto and Andrea Patriarca 3. Aspergillus Species and Their Associated Mycotoxins Giancarlo Perrone and Antonia Gallo 4. Fusarium Species and Their Associated Mycotoxins Gary P. Munkvold 5. Penicillium Species and Their Associated Mycotoxins Giancarlo Perrone and Antonia Susca Part II: Polymerase Chain Reaction (PCR)-Based Methods for Detection and Identification of Mycotoxigenic Fungi 6. Targeting Conserved Genes in Alternaria Species Miguel ngel Pavn, Ins Mara Lpez-Calleja, Isabel Gonzlez, Rosario Martn, and Teresa Garca 7. Targeting Conserved Genes in Aspergillus Species Sndor Kocsub and Jnos Varga 8. Targeting Conserved Genes in Fusarium Species Jssica Gil-Serna, Beln Patio, Miguel Jurado, Salvador Mirete, Covadonga Vzquez, and M. Teresa Gonzlez-Jan 9. Targeting Conserved Genes in Penicillium Species Stephen W. Peterson 10. Targeting Aflatoxin Biosynthetic Genes Ali Y. Srour, Ahmad M. Fakhoury, and Robert L. Brown 11. Targeting Trichothecene Biosynthetic Genes Songhong Wei, Theo van der Lee, Els Verstappen, Marga van Gent, and Cees Waalwijk 12. Targeting Ochratoxin Biosynthetic Genes Antonia Gallo and Giancarlo Perrone 13. Targeting Fumonisin Biosynthetic Genes Robert H. Proctor and Martha M. Vaughan 14. Targeting Other Mycotoxin Biosynthetic Genes Mara J. Andrade, Mar Rodrguez, Juan J. Crdoba, and Alicia Rodrguez 15. Evaluating Aflatoxin Gene Expression in Aspergillus Section Flavi Paula Cristina Azevedo Rodrigues, Jessica Gil-Serna, and M. Teresa Gonzlez-Jan 16. Evaluating Fumonisin Gene Expression in Fusarium verticillioides Valeria Scala, Ivan Visentin, and Francesca Cardinale Part III: Polymerase Chain Reaction (PCR)-Based Methods for Multiplex Detection of Mycotoxigenic Fungi 17. Multiplex Detection of Aspergillus Species Pedro Martnez-Culebras, Mara Victoria Selma, and Rosa Aznar 18. Multiplex Detection of Fusarium Species Tapani Yli-Mattila, Siddaiah Chandra Nayaka, Mudili Venkataramana, and Emre Yrk 19. Multiplex Detection of Penicillium Species Alicia Rodrguez, Juan J. Crdoba, Mar Rodrguez, and Mara J. Andrade Part IV: Combined PCR and Other Molecular Approaches for Detection and Identification of Mycotoxigenic Fungi 20. PCR-RFLP for Aspergillus Species Ali Atoui and Andr El Khoury 21. PCR ITS-RFLP for Penicillium Species and Other Genera Sandrine Rousseaux and Michle Guilloux-Bnatier Part V: New Methodologies for Detection and Identification of Mycotoxigenic Fungi
