Description
This book provides a collection of microphysiological systems employed for chemical/drug screening and strategies to mimic various physiological conditions. Chapters guide readers through Organ-on-a-Chip( OoC) platforms such as liver, intestine, blood-brain barrier, kidney, vessels, cardiac and skeletal muscles, articular joint, human fat. Additional chapters detail microfabrication technologies used to fabricate OoC devices such as, standard photo- and soft-lithography, techniques to fabricate membranes, and industrial-oriented fabrication methods.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Organ-On-a Chip: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 1. Photo and Soft Lithography for Organs-on-Chip applications Erika Ferrari, Federico Nebuloni, Marco Rasponi, and Paola Occhetta 2. Organs-on-chips for Studying Tissue Barriers: Standard Techniques and a Novel Method for Including Porous Membranes Within Microfluidic Devices Mattia Ballerini, Mohammad Jouybar, Andrea Mainardi, Marco Rasponi, and Giovanni Stefano Ugolini 3. Thermoplastic microfluidics Per Magnus Kristiansen, Agnieszka Karpik, Jerome Werder, Marco Guilherme, and Michael Grob 4. Bile Duct-On-a-Chip Yu Du, William J. Polacheck, and Rebecca G. Wells 5. Establishment of a Modular Anaerobic Human Intestine Chip Sasan Jalili-Firoozinezhad, Amir Bein, Francesca S. Gazzaniga, Cicely W. Fadel, Richard Novak, and Donald E. Ingber 6. Intestinal Epithelium Tubules on a Chip Kinga Kosim, Iris Schilt, Henriette L Lanz, Paul Vulto, and Dorota Kurek 7. A Human Neurovascular Unit On-a-chip Sharon Wei Ling Lee, Renato Rogosic, Claudia Venturi, Manuela Teresa Raimondi, Andrea Pavesi, and Giulia Adriani 8. Generation of a Glomerular Filtration Barrier on a Glomerulus-on-a-chip Platform Laura Perin, and Stefano Da Sacco 9. Electro-Mechanical Stimulation of 3D Cardiac Microtissues in a heart-on-chip Model Roberta Visone, Paola Occhetta, and Marco Rasponi 10. Miniaturized Vascularized Bleeding Model of Hemostasis Elaissa Trybus Hardy, Yumiko Sakurai, and Wilbur A. Lam 11. Fabrication and Use of a Pumpless Microfluidic Lymphatic Vessel Chip Parinaz Fathi and Mandy B. Esch 12. On Chip-Platelet Activation Assessment: Microfluidic Emulation of Shear Stress Profiles Induced by Mechanical Circulatory Support Devices Tatiana Mencarini, Silvia Bozzi, and Alberto Redaelli 13. Biofabrication of 3D Human Muscle Model with Vascularization and Endomysium Simone Bersini, Riccardo Francescato, and Matteo Moretti 14. Mechanical induction of Osteoarthritis traits in a cartilage-on-chip model Andrea Mainardi, Paola Occhetta, and Marco Rasponi 15. A Synovium/Cartilage-On-A-Chip Model To Investigate The Process Of Monocyte Extravasation Silvia Palombella, Shima Salehi, Carlotta Mondadori, Giuseppe Tal, Valerio Sansone, Matteo Moretti, and Silvia Lopa 16. A Mesoscale 3D Culture System For Native And Engineered Biphasic Tissues: Application To The Osteochondral Unit Irene Chiesa, Roberto Di Ges, Kalon J. Overholt, and Riccardo Gottardi 17. 3D biofabricated in Vitro Models of Vascularized and Mineralized Bone Tissues Maria Vittoria Colombo, Simone Bersini, Chiara Arrigoni, and Matteo Moretti 18. Isolation, Integration, and Culture of Human Mature Adipocytes Leveraging Organ-On-Chip Technology Julia Rogal, Julia Roosz, and Peter Loskill
