Description
This second edition volume expands on the previous edition with new information on the latest developments in the field, and the most up-to-date representative methodological information on molecular neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters in this book are organized into three parts. Part One discusses molecular immunological and pharmacological aspects related to the detection of receptors and ion channels in the brain. Part Two explores the neuroanatomical techniques devoted to localizing receptors and ion channels within the central nervous system. Part Three looks at functional approaches devoted to ascertaining receptors and ion channels functionality in the brain. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Receptor and Ion Channels Detection in the Brain, Second Edition is a valuable resource that contains detailed molecular, cellular, neuroanatomical, and electrophysiological methods that will help researchers study neurotransmitter receptor and ion channel in any region of neuron of the brain. Series Preface… Preface… Table of Contents… Contributing Authors… Part I Molecular Techniques 1. Production of High-Quality Antibodies for the Study of Receptors and Ion Channels Masahiko Watanabe 2. Co-Immunoprecipitation from Brain Xavier Morat, Dasiel O. Borroto-Escuela, Kjell Fuxe, Vctor Fernndez-Dueas, and Francisco Ciruela 3. Sub-Synaptic Membrane Fractionation Paula M. Canas and Rodrigo A. Cunha 4. Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation Elek Molnr 5. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells Juan Varela, Julien Dupuis, Franois Maingret, and Laurent Groc 6. Radioligand Binding Detection of Receptors in Brain Membranes Fuencisla Pilar-Cullar, Emilio Garro-Martnez, Elena Castro, and lvaro Daz 7. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain Markus U. Ehrengruber and Kenneth Lundstrom 8. Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain Vctor Fernndez-Dueas, Thierry Durroux, and Francisco Ciruela 9. Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay Manuel Narvez, Minerva Crespo-Ramrez, Ramn Fores-Pons, Mariana Pita-Rodrguez, Francisco Ciruela, Malgorzata Filip, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Miguel Perezde la Mora, Kjell Fuxe, and Dasiel O. Borroto-Escuela 10. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain Marta Valle-Len, Vctor Fernndez-Dueas, and Francisco Ciruela Part II Neuroanatomical Techniques 11. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling Miwako Yamasaki and Masahiko Watanabe 12. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain Rebeca Vidal, Fuencisla Pilar-Cuellar, lvaro Daz, and Elena Castro 13. Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting Elek Molnr 14. Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility Kohtarou Konno and Masahiko Watanabe 15. Localization of GFP-Tagged Proteins at the Electron Microscope Sara Gil-Perotin, A. Cebrin-Silla, V. Herranz-Prez, P. Garca-Belda, S. Gil-Garca, M. Fil, J.S. Lee, M.V. Nachury, and Jos Manuel Garca-Verdugo 16. Tyramide Signal Amplificacin for Immunoelectron Microscopy Mara Jos Ulloa-Navas, Patricia Garca-Trraga, Susana Gonzlez-Granero, Pedro Prez-Borreda, Vicente Herranz-Prez, and Jos Manuel Garca-Verdugo 17. Pre-Embedding Methods for the Localization of Receptors and Ion Channels Rafael Lujn 18. Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels Rafael Lujn and Masahiko Watanabe 19. High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL) Walter A. Kaufmann, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto 20. Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM Marta Turgano-Lpez, Jos Rodrigo-Rodrguez, Lidia Alonso-Nanclares, Juncal Gonzlez-Soriano, Javier DeFelipe, and ngel Merchn-Prez 21. Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta 22. Efficient Labeling of Neurons and Identification of Postsynaptic Sites Using Adeno-Associated Virus Vector Megumu Takahashi, Yoko Ishida, Naoya Kataoka, Kazuhiro Nakamura, and Hiroyuki Hioki 23. Analysis of Synaptic Connections at the Electron Microscopic Level using Sindbis Virus Vectors Takahiro Furuta, Keiko Okamoto-Furuta, and Hiroyuki Hioki 24. Morphological and Neurochemical Characterization of Electrophysiologically Identified Cells Yoshiyuki Kubota Part III Functional Techniques 25. Using Electrophysiology to Study Synaptic and Extrasynaptic Ionotropic Receptors in Hippocampal Neurons Ian D. Coombs and David Soto 26. Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels Norbert Weiss and Michel De Waard 27. Electrophysiological Recordings in Behaving Animals Agns Gruart and Jos M. Delgado-Garca 28. Voltammetry in Behaving Animals Kendra D. Bunner and George V. Rebec 29. In Vivo Brain Microdialysis of Monoamines Jorge E. Ortega, Blanca Prez-Palomar, J. Javier Meana, and Luis F. Callado 30. Optical Control of Brain Receptors using Photoactive Drug in Behaving Animals Marc Lpez-Cano, Vctor Fernndez-Dueas, and Francisco Ciruela 31. Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor Vctor Fernndez-Dueas, Xavier Morat, Thomas Knpfel, and Francisco Ciruela Chapter 32. Monitoring GPCR-Mediated cAMP Accumulation in Rat Striatal Synaptosomes Jaume Taura, Vctor Fernndez-Dueas, and Francisco Ciruela 33. GPCR-Mediated MAPK/ERK Cascade Activation in Mouse Striatal Slices Maricel Gmez-Soler, Vctor Fernndez-Dueas, and Francisco Ciruela Subject Index List…
