Description
This book presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. It is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general. Each chapter provides a short overview of the topic, followed by detailed explanations of techniques. Table of Contents1. Quantitative, Multiplexed Detection of Salmonella and OtherPathogens byLuminexSherry A. Dunbar and James W. Jacobsen2. Detection of Salmonella by Bacteriophage Felix 01Jonathan C. Kuhn3. Physical Mapping of Salmonella GenomesShu-Lin Liu4. Cloth-based Hybridization Array System for the Identification ofAntibioticResistance Genes in SalmonellaBurton W. Blais and Martine Gauthier5. Genome-based Identification of Molecular Analyses of PathogenicityIslands andGenomic Islands in Salmonella entericaMichael Hensel6. Determination of the Gene Content of Salmonella Genomes by MicroarrayAnalysisSteffen Porwollik and Michael McClelland7. In-Vivo Excision, Cloning, and Broad-Host-Range Transfer of LargeBacterialDNA Segments Using VEX-CaptureJames A. Wilson and Cheryl A. Nickerson8. Amplified Fragment Length Polymorphism Analysis of SalmonellaentericaRuiting Lan and Peter R. Reeves9. Salmonella Phages and Prophages – Genomics and Practical AspectsAndrew M. Kropinski, Alexander Sulakvelidze, Paulina Konczy, andCorneliusPoppe10. Salmonella Typhimurium Phage Typing for PathogensWolfgang Rabsch11. Salmonella Phages Examined in the Electron MicroscopeHans-W. Ackermann12. Applications of Cell Imaging in Salmonella ResearchCharlotte A. Perrett and Mark A. Jepson13. Analysis of Kinesin Accumulation on Salmonella-containing VacuolesAudrey Dumont, Nina Schroeder, Jean-Pierre Gorvel, and StphaneMresse14. Magnesium, Manganese and Divalent Cation Transport Assays in IntactCellsMichael E. Maguire15. Methods in Cell-to-Cell Signaling in SalmonellaBrian M. M. Ahmer, Jenee N. Smith, Jessica L. Dyszel, and AmberLindsay16. Development of Salmonella Strains as Cancer Therapy Agents andTesting inTumor Cell LinesAbraham Eisenstark, Robert A. Kazmierczak, Alison Fea, Rula Khreis,DustinNewman, and Heide Schatten17. Further Resources for Molecular Protocols in Salmonella ResearchAbraham Eisenstark and Kelly K. Edwards
