Description
This methods book covers novel approaches and methods that have been developed to address the specific features of signaling by cell adhesion molecules. It brings together the current methodology employed in studies of signaling by cell adhesion molecules and blends excellent descriptions and applications of well-established methods at the cutting edge of signal transduction research. TOC:Protein-Protein Interactions and Early Events in Integrin Signaling: The Use of Chimeric Receptors in the Study of Integrin Signaling. Using Synthetic Peptides to Mimic Integrins: Probing Cytoskeletal Interactions and Signaling Pathways. Use of the Yeast Two-Hybrid System for the Characterization of Integrin Signal Transduction Pathways: Identification of the Integrin-Linked Kinase, p59. Focal Adhesion Kinase and its Associated Proteins. Analysis of Paxillin as a Multi-Domain Scaffolding Protein. p130 in Integrin Signaling. Specificity of Integrin Signaling.- Late Events and Biological Functions of Integrin Signaling: Methods for Study of Integrin Regulation of MAP Kinase Cascades. Methods for Analysis of Adhesion-Dependent Cell Cycle Progression. Integrin Modulation of Mitogenic Pathways Involved in Muscle Differentiation. Methods for Studying Anoikis. Functional Analysis of FAK and Associated Molecules in Cell Migration. Integrin Signaling in Pericellular Matrix Assembly.- Inside-out Signaling by Integrins: Studies of Integrin Signaling Through Platelet ab. Tracking of Integrin-Mediated Adhesion Using Green Fluorescent Protein and Flow Cytometry. Expression Cloning of Proteins That Modify Integrin Activation. Regulation of Cell Contractility by RhoA: Stress Fiber and Focal Adhesion Assembly.- Studies of Integrin Signaling Through Platelet General Methods for Studies of Cell Adhesion Molecule-Mediated Signaling: Intra- and Intercellular Localization of Proteins in Tissue in situ. Optical Microscopy Studies of (Ca2) signaling. Wnt Signaling in Xenopus Embryos.
