Description
1. How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating Steven E. Mansoor 2. Agonists, Antagonists, and Modulators of P2X7 Receptors Christa E. Mller and Vigneshwaran Namasivayam 3. Modulation of Cell Energy Metabolism by the P2X7 Receptor Francesco Di Virgilio, Simonetta Falzoni, Alba Clara Sarti, Paola Chiozzi, Valentina Vultaggio-Poma, and Anna Lisa Giuliani 4. Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE Sudad Dayl and Ralf Schmid 5. Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4) Amal Elhage, Ross J. Turner, Peter Cuthbertson, Debbie Watson, and Ronald Sluyter 6. Development of Antibody and Nanobody Tools for P2X7 Tobias Sthler, Welbeck Danquah, Melanie Demeules, Henri Gond, Romain Hardet, Friedrich Haag, Sahil Adriouch, Friedrich Koch-Nolte, and Stephan Menzel 7. Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors Anna Marei Mann, Waldemar Schfer, Sahil Adriouch, Kathleen Brner, Dirk Grimm, Ingke Braren, and Friedrich Koch-Nolte 8. Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations Tina Jooss, Birgit Kuch, Jiong Zhang, Michael Schemann, and Annette Nicke 9. Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes Gnther Schmalzing and Fritz Markwardt 10. A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors Anna Durner and Annette Nicke 11. Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents Xin Liang, Laura Janks, and Terrance M. Egan 12. Using Symmetrical Organic Cation Solutions to Study P2X7 Ion Permeation Kate Dunning, Laurie Peverini, and Thomas Grutter 13. Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors Ruth D. Murrell-Lagnado and Toshimitsu Kawate 14. Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry Samantha Wittke, Leif Ancker, Sana Javed, and Friedrich Haag 15. Characterization of P2X7 Receptors in Human Blood Cells Laura Hurtado-Navarro, Alberto Baroja-Mazo, and Pablo Pelegrn 16. Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube Alexander W. Veltkamp, Tim Magnus, and Bjrn Rissiek 17. Administration of P2X7 Receptor Blockers in Oncological Experimental Models Elena De Marchi, Anna Pegoraro, and Elena Adinolfi 18. Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease Debbie Watson, Sam R. Adhikary, Peter Cuthbertson, Nicholas J. Geraghty, Katrina M. Bird, Amal Elhage, Chloe Sligar, and Ronald Sluyter 19. Analysis of P2X7-Induced Neuronal Branching Paula Mut-Arbona and Beta Sperlgh 20. Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique lvaro Sebastin-Serrano, Carolina Bianchi, Caterina Di Lauro, Lucia Soria-Tobar, Beatriz lvarez-Castelao, and Miguel Daz-Hernndez 21. Analyzing the Role of the P2X7 Receptor in Epilepsy Mariana Alves, Laura de Diego-Garcia, and Tobias Engel
