Description
This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 2: Vaccines for Veterinary Diseases includes vaccines for farm animals and fishes, vaccine vectors and production, vaccine delivery systems, vaccine bioinformatics, vaccine regulation and intellectual property. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases aims to ensure successful results in the further study of this vital field. Part I Challenges in Veterinary Vaccine Development 1. Challenges in Veterinary Vaccine Development and Immunization Mark A. Chambers, Simon P. Graham, and Roberto M. La Ragione Part II Vaccines for Farm Animals 2. Development of Mycoplasma hyopneumoniae Recombinant Vaccines Silvana Beutinger Marchioro, Simone Simionatto, and Odir Dellagostin 3. Computational Prediction of Immunodominant Epitopes on Outer Membrane Protein (Omp) H of Pasteurella Multocida towards Designing of a Peptide Vaccine Bhaskar Ganguly 4. DNA Vaccines against Maedi-Visna Virus Ana M. Henriques, Miguel Fevereiro, and Gabriel A. Monteiro 5. Detection of Avian Antigen-Specific T cells Induced by Viral Vaccines Tina Srensen Dalgaard, Liselotte Rothmann Norup, and Helle Risdahl Juul-Madsen 6. Generation of Newcastle Disease Virus (NDV) Recombinants Expressing the Infectious Laryngotracheitis Virus (ILTV) Glycoprotein gB or gD as Dual Vaccines Wei Zhao, Stephen Spatz, Laszlo Zsak, and Qingzhong Yu 7. A Reverse Genetics Approach for the Design of Methyltransferase-Defective Live Attenuated Avian Metapneumovirus Vaccines Yu Zhang, Jing Sun, Yongwei Wei, and Jianrong Li 8. Development of Fasciola Vaccine in an Animal Model Krai Meemon, and Pras ert Sobhon 9. Development of Experimental Vaccines against Liver Flukes Huan Yong Yap and Peter M. Smooker 10. Towards a Preventive Strategy for Toxoplasmosis: Current Trends, Challenges, and Future Perspectives for Vaccine Development Ragab M. Fereig and Yoshifumi Nishikawa 11. DNA V accination in Chickens Shishir Kumar Gupta, Sohini Dey, and Madhan Mohan Chellappa PART III Vaccines for Fishes and Shellfish 12. Selection of Vaccine Candidates for Fish Pasteurellosis Using a Reverse Vaccinology and an In Vitro Screening Approach Francesca Andreoni, Giulia Amagliani, and Mauro Magnani 13. Development of Vaccines against Nocardia in Fishes Sukanta K. Nayak and Teruyuki Nakanishi 14. Design of an Immersion Vaccine against Aeromonad Septicaemia in Perch (Perca fluviatilis L.). Joachim Frey, Sarah E. Burr, and Thomas Wahli 15. Prokaryotic Production of Virus-Like Particle Vaccine of Betanodavirus Junfeng Xie, Runqing Huang, and Yuxiong Lai 16. Design an d Construction of Shrimp Antiviral DNA Vaccines Expressing Long and Short Hairpins for Protection by RNA Interference Aparna Chaudhari, Gireesh Babu P, and A. Pavan Kumar PART IV Vaccines against Ticks 17. Developing Anti-Tick Vaccines Al ina Rodrguez-Mallon 18. Host Immunization with Recombinant Proteins to Screen Antigens for Tick Control Remil Linggatong Galay, Takeshi Miyata, Rika Umemiya-Shirafuji, Masami Mochizuki, Kozo Fujisaki, and Tetsuya Tanaka 19. Vaccinomics Approach to Tick Vaccine Development Marinela Contreras, Margarita Villar, Pilar Alberdi, and Jos de la Fuente Part V Vaccine Adjuvants 20. Development of CpG ODN Based Vaccine Adjuvant Formulations Mayda Gursel and Ihsan Gursel Part VI Vaccine Vectors and Producti on Systems 21. Assembly and Assessment of DNA Scaffolded Vaccines Xiaowei Liu, Lili Wang, Hao Yan, and Yung Chang 22. Alphavirus-based Vaccines Kenneth Lundstrom 23. Vaccine Design: Replication-Defective Adenovirus Vectors Xiangyang Zhou, Zhiquan Xiang, and Hildegund C.J. Ertl 24. Generation of Lymphocytic Choriomeningitis Virus based Vaccine Vectors Sandra Ring and Lukas Flatz 25. Production of Japanese Encephalitis Virus-like Particles Using Insect Cell Expression Systems Hideki Yamaji and Eiji Konishi 26. Subunit Protein Vaccine Delivery System for Tuberculosis Based on Hepatitis B Virus Core VLP (HBc-VLP) Particles Dhananjayan Dhanasooraj, Ajay Kumar R, and Sathish Mundayoor 27. Formulation Studies during Preclinical Development of Influenza Hemagglutinin and Virus-like Particle Vaccine Candidates Newton Wahome, John M. Hickey, David B. Volkin, and C. Russell Middaugh 28. Strategies for Vaccine Design using Phage Display-Derived Peptides Luiz R. Gou lart and Paula de S. Santos 29. Production of Well-Characterized Virus-Like Particles in an Escherichia coli based Expression Platform for Preclinical Vaccine Assessments Newton Wahome, Anne Cooper, Prem Thapa, Shyamal Choudhari, Fei P. Gao, David B. Volkin, and C. Russell Middaugh 30. Laboratory Scale Production of Recombinant Haa86 Tick Protein in Pichia pastoris and in Escherichia coli System Binod Kumar, Azhahianambi P, and Ghosh Srikant 31. Production and Purification of Recombinant Filamentous Bacteriophages Displaying Immunogenic Heterologous Epitopes Lei Deng, Florencia Linero, and Xavier Saelens 32. Oral Vaccine Development by Molecular Display Methods using Microbial Cells Seiji Shibasaki and Mitsuyoshi Ueda 33. Facile Method for the Production of Recombinant Cholera Toxin B Subunit in E. Coli Krystal Hamorsky and Nobuyuki Matoba 34. Immunoproteomic Approach for Screening Vaccine Candidates from Bacterial Outer Membrane Proteins Jianyi Pan, Chuchu Li, and Zhicang Ye 35. Construction and Immunogenicity Testing of Whole Recombinant Yeast-Based T cell Vaccines Thomas H. King, Zhimin Guo, Melanie Hermreck, Donald Bellgrau, and Timothy C. Rodell 36. Oral Rabies Vaccine Design For Expression in Plants Ankit Singh, Gauri Saxena, and Praveen C. Verma 37. Purification of Virus-like Particles (VLPs) from Plants Albertha R. van Zyl, and Inga I. Hitzeroth 38. Transient Expression of Viral Proteins in Plants using Agrobacterium tumefaciens Inga I. Hitzeroth, and Albertha R. van Zyl 39. A Plant-Based Transient Expression System for the Rapid Production of Malaria Vaccine Candidates Alexander Boes, Andreas Reimann, Richard M Twyman, Rainer Fischer, Stefan Schillberg, and Holger Spiegel 40. Recombinant Botulinum Toxoids: A Practical Guide for Production Gustavo Maral S.G. Moreira, Clvis Moreira Jr., Carlos Eduardo P. da Cunha, Marcelo Mendona, and Fabricio R. Conceio 41. Practical Approaches to Forced Degradation Studies of Vaccines Manvi Hasija, Sepideh Aboutorabian, Nausheen Rahman, and Salvador F Ausar PART VII Vaccine Delivery Systems 42. Preparation of Multifunctional Liposomes as a Stable Vaccine Delivery-Adjuvant System by Procedure of Emulsification-Lyophilization Tin g Wang, and Ning Wang 43. Preparation of the Multifunctional Liposome-Containing Microneedle Arrays as an Oral Cavity Mucosal Vaccine Adjuvant-Delivery System Ting Wang, and Ning Wang 44. Preparation and Characterization of PLGA Encapsulated Protective Antigen Domain 4 Nanoformulation Manish Manish, Rakesh Bhatnagar, and Samer Singh 45. Attenuated Salmonella sp. as a DNA Delivery Dystem for T. cruzi Antigens Augusto E. Bivona, Natacha Cerny, Andrs Snchez Alberti, Silvia I. Cazorla, and Emilio L. Malchiodi 46. Poly-e-Caprolactone/Chitosan and Chitosan Particles: Two Recombinant Antigen Delivery Systems for Intranasal Vaccination Sandra Jesus, Edna Soares, and Olga Borges 47. Micro-Fractional Epidermal Powder Delivery for Skin Vaccination Feng Jia, Shengwu Liu, Mei X. Wu, and Xinyuan Chen Part VIII Vaccine Bioinformatics 48. A Bioinformatics Method for the Design of Live Attenuated Virus Vaccine Utilizing Host microRNA Response Elements Duangdao Wichadakul 49. The Web-based DNA Vaccine Dat abase DNAVaxDB and its Usage for Rational DNA Vaccine Design Rebecca Racz and Yongqun He 50. MetaMHCpan, a Meta Approach for Pan-Specific MHC Peptide Binding Prediction Yichang Xu, Cheng Luo, Hiroshi Mamits uka, and Shanfeng Zhu 51. A Cohesive and Integrated Platform for Immunogenicity Prediction Ivan Dimitrov, Mariyana Atanasova, Atanas Patronov, Darren R. Flower, and Irini Doytchinova Part IX Vaccine Safety and Regulation 52. The Regulatory Evaluation of Vaccines for Human Use Norman W. Baylor PART X Vaccine Intellectual Property 53. Vaccines and IP Rights: A Multifaceted Relationship Karen Durell 54. From the Bench to the Pharmacy: Protecting Innovation during Vaccine Development and Commercialization Adam C. Krol, Muna Abu-Shaar, and Paul Brady 55. Intellect ual Property in Vaccine Innovation: Impact of Recent Patent Developments Elizabeth Siew-Kuan Ng
